Fat that is collected with the intention of transferring it to another body area is generally collected by process of liposuction. Fat is most commonly harvested from the tummy or thigh regions (as they are easily accessible).
For Micro-fat transfer, liposuction is done using a 2-3 mm cannula (metal tube) with multiple small holes (1mm diameter) near the tip. This ensures that the fat particle size is not more than 1 mm. This fat is then processed as per your surgeon’s preference by an open or closed technique. I prefer the closed technique where I allow the lipoaspirate to sediment and then centrifuge it to isolate the fat component to be transferred.
For preparing nano-fat, the above lipoaspirate is mechanically processed to emulsify the tissue, destroy the fat cells and retain only the stem cells.
This is done by a systematic process of to-and-fro passage of the fat from one syringe to another via a sequentially narrowing connector (adaptor). This ensures that only “stem cells” are surviving in the tissue. This “fluid” form of fat is then filtered over a special filter to remove any unwanted cell debris and fibrous tissue. The fluid that comes out is nanofat!
This process takes approximately 15-20 minutes and can be done as an office procedure if small volumes are to be transferred.
Since the stem cells are merely isolated by a mechanical process there are no restrictions to its clinical use.